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KMID : 0043320060290080617
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Archives of Pharmacal Research
2006 Volume.29 No. 8 p.617 ~ p.623
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Chemical Constituents of the Root of Dystaenia takeshimana and Their Anti-Inflammatory Activity
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Kim Ju-Sun
Kim Jin-Cheul
Shim Sang-Hee
Lee Eun-Ju
Jin WenYi
Bae Ki-Hwan
Son Kun-Ho
Kim Hyun-Pyo
Kang Sam-Sik
Chang Hyun-Wook
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Abstract
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In our ongoing search for bioactive compounds originating from the endemic species in Korea, we found that the hexane and EtOAc fractions of the MeOH extract from the root of Dystaenia takeshimana (Nakai) Kitagawa (Umbelliferae) showed cyclooxygenase-2 (COX-2) and 5- lipoxygenase (5-LOX) dual inhibitory activity by assessing their effects on the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) in mouse bone marrow-derived mast cells. By activity-guided fractionation, five coumarins, viz. psoralen (2), xanthotoxin (3), scopoletin (4), umbelliferone (5), and (+)-marmesin (6), together with ?sitosterol (1), were isolated from the hexane fraction, and two phenethyl alcohol derivatives, viz. 2-methoxy-2-(4’-hydroxyphenyl) ethanol (7) and 2-hydroxy-2-(4’-hydroxyphenyl)ethanol (8), three flavonoids, viz. apigenin (9), luteolin (10), and cynaroside (11), as well as daucosterol (12) were isolated from the EtOAc fraction using silica gel column chromatography. In addition, D-mannitol (13) was isolated from the BuOH fraction by recrystallization. Two of the coumarins, scopoletin (4) and (+)- marmesin (6), the two phenethyl alcohol derivatives (7, 8) and the three flavonoids (9?1) were isolated for the first time from this plant. Among the compounds isolated from this plant, the five coumarins as well as the three flavonoids showed COX-2/5-LOX dual inhibitory activity. These results suggest that the anti-inflammatory activity of D. takeshimana might in part occur via the inhibition of the generation of eicosanoids.
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KEYWORD
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Dystaenia takeshimana, Umbelliferae, Chemical constituents, Cyclooxygenase-2, 5-Lipoxygenase, Anti-inflammatory activity
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